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Standardize Your Small Particle Flow Cytometry

FCMPASS is a comprehensive software for cataloguing, optimizing, calibrating, and reporting small particle flow cytometry data — improving rigor and reproducibility across instruments and laboratories.

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FCMpass Workflow

Data adapted from: Cook et al., 2023, Cell Reports Methods 3, 100664. DOI: 10.1016/j.crmeth.2023.100664

What FCMPASS Does

  • Catalogue

    Store and manage your flow cytometers, light scatter reference materials, fluorescent beads, and rainbow particles in a centralized catalogue — reducing repetitive data entry and ensuring consistency.

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  • Calibrate

    Calibrate fluorescence and light scatter data into standardized units, enabling cross-platform comparisons and traceable MIFlowCyt and MIFlowCyt-EV reports.

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  • Optimize

    Generate and analyze detector optimization data for both fluorescence and light scatter channels to get the best performance from your flow cytometer.

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  • Report

    Automatically generate transparent, shareable reports with detailed metadata — ready for publication and compliant with MIFlowCyt-EV guidelines.

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Used by Researchers Worldwide

FCMPASS has been used by 600+ researchers across 29 countries and demonstrated in peer-reviewed publications by the software's author in journals including Cytometry Part A, Cell Reports Methods, Nano Letters, and Journal of Extracellular Vesicles.

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Supported Cytometer Platforms

FCMPASS works with instruments from leading manufacturers:

Agilent · Beckman Coulter · Waters (formerly Becton Dickinson) · Cytek Biosciences · nanofcm · Sony Biosciences · ThermoFisher Scientific

Full cytometer list

Get Started

  • Download

    Available for Windows and macOS (Apple Silicon). Free for qualifying academic institutions.

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  • Pricing

    Free academic licenses and paid commercial tiers.

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